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plates  (Greiner Bio)


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    Greiner Bio plates
    Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 2473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plates+greiner+bio+one+cellstar+96+well+white+cell+culture+treated+flat+bottom+microplate+655098/Cell+Culture+Microplate+96+Well+Ps+F-Bottom/pmc12168084-118-33-34
    Average 99 stars, based on 2473 article reviews
    plates - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: A flexible, high-throughput system for studying live mRNA translation with HiBiT technology
    Article Snippet: .. Cells were reverse-transfected with 1.2 pmol of mRNA with Lipofectamine RNAiMAX Transfection Reagent according to the manufacturer’s instructions and plated on clear bottom, white-walled plates (Greiner Bio-One CELLSTAR 96-well, White, Cell Culture-Treated, Flat-Bottom Microplate, 655098) at 50 000 cells/well in appropriate media (RPMI for A549, CDM-BSA for hESC-CMs) and incubated at 37°C. .. After 4 h, cells were washed with Dulbecco's Phosphate Buffered Saline (DPBS) (Sigma, D8537) and lysed with lytic buffer composed of 0.05% Triton X-100 (VWR, 0694), LgBiT (1:200, Promega, N112A), and Furimazine (1:100 Promega, N113A) in DPBS.

    Article Title: A flexible, high-throughput system for studying live mRNA translation with HiBiT technology.
    Article Snippet: .. In cellulo HiBiT endpoint lytic assay Cells were reverse-transfected with 1.2 pmol of mRNA with Lipofectamine RNAiMAX Transfection Reagent according to the manufacturer’s instructions and plated on clear bottom, white-walled plates (Greiner Bio-One CELLSTAR 96- well, White, Cell Culture-Treated, Flat-Bottom Microplate, 655098) at 50 000 cells / well in appropriate media (RPMI for A549, CDM-BSA for hESC-CMs) and incubated at 37 ◦C. .. After 4 h, cells were washed with Dulbecco’s Phosphate Buffered Saline (DPBS) (Sigma, D8537) and lysed with lytic buffer composed of 0.05% Triton X-100 (VWR, 0694), LgBiT (1:200, Promega, N112A), and Furimazine (1:100 Promega, N113A) in DPBS.

    Article Title: A flexible, high-throughput system for studying mRNA translation kinetics in vitro and in cellulo with HiBit technology
    Article Snippet: HEK293 LgBit cells (gift from Itzhaki lab) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher, 41966052) supplemented with L-glutamine (Thermo Fisher, 25030-024) and 10% FBS (Sigma, F7524). .. For live cell experiments of mRNA translation kinetics, HEK293 LgBit cells were reverse-transfected with 1.2 pmol of mRNA with Lipofectamine RNAiMAX Transfection Reagent according to the manufacturer’s instructions and plated on clear bottom, white-walled plates (Greiner Bio-One CELLSTAR 96-well, White, Cell Culture- Treated, Flat-Bottom Microplate, 655098) at 40, 000 cells/well in Leibovitz’s L-15 Medium no phenol red (ThermoFisher, 21083-027), which supports cell growth in environments without CO2 equilibration, supplemented with 10% FBS and substrate Nano-Glo Endurazine Live Cell Substrate (Promega, N2570). ..

    Article Title: A flexible, high-throughput system for studying live mRNA translation with HiBiT technology
    Article Snippet: .. For live-cell experiments of mRNA translation dynamics, HEK293 LgBiT cells were reverse-transfected with 1.2 pmol of mRNA with Lipofectamine RNAiMAX Transfection Reagent according to the manufacturer’s instructions and plated on clear bottom, white-walled plates (Greiner Bio-One CELLSTAR 96-well, White, Cell Culture-Treated, Flat-Bottom Microplate, 655098) at 40 000 cells/well in Leibovitz’s L-15 Medium no phenol red (Thermo Fisher, 21083-027), which supports cell growth in environments without CO 2 equilibration, supplemented with 10% FBS and substrate Nano-Glo Endurazine Live Cell Substrate (Promega, N2570, 1:200). ..

    Incubation:

    Article Title: A flexible, high-throughput system for studying live mRNA translation with HiBiT technology
    Article Snippet: .. Cells were reverse-transfected with 1.2 pmol of mRNA with Lipofectamine RNAiMAX Transfection Reagent according to the manufacturer’s instructions and plated on clear bottom, white-walled plates (Greiner Bio-One CELLSTAR 96-well, White, Cell Culture-Treated, Flat-Bottom Microplate, 655098) at 50 000 cells/well in appropriate media (RPMI for A549, CDM-BSA for hESC-CMs) and incubated at 37°C. .. After 4 h, cells were washed with Dulbecco's Phosphate Buffered Saline (DPBS) (Sigma, D8537) and lysed with lytic buffer composed of 0.05% Triton X-100 (VWR, 0694), LgBiT (1:200, Promega, N112A), and Furimazine (1:100 Promega, N113A) in DPBS.

    Article Title: A flexible, high-throughput system for studying live mRNA translation with HiBiT technology.
    Article Snippet: .. In cellulo HiBiT endpoint lytic assay Cells were reverse-transfected with 1.2 pmol of mRNA with Lipofectamine RNAiMAX Transfection Reagent according to the manufacturer’s instructions and plated on clear bottom, white-walled plates (Greiner Bio-One CELLSTAR 96- well, White, Cell Culture-Treated, Flat-Bottom Microplate, 655098) at 50 000 cells / well in appropriate media (RPMI for A549, CDM-BSA for hESC-CMs) and incubated at 37 ◦C. .. After 4 h, cells were washed with Dulbecco’s Phosphate Buffered Saline (DPBS) (Sigma, D8537) and lysed with lytic buffer composed of 0.05% Triton X-100 (VWR, 0694), LgBiT (1:200, Promega, N112A), and Furimazine (1:100 Promega, N113A) in DPBS.

    Cell Culture:

    Article Title: A flexible, high-throughput system for studying mRNA translation kinetics in vitro and in cellulo with HiBit technology
    Article Snippet: HEK293 LgBit cells (gift from Itzhaki lab) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher, 41966052) supplemented with L-glutamine (Thermo Fisher, 25030-024) and 10% FBS (Sigma, F7524). .. For live cell experiments of mRNA translation kinetics, HEK293 LgBit cells were reverse-transfected with 1.2 pmol of mRNA with Lipofectamine RNAiMAX Transfection Reagent according to the manufacturer’s instructions and plated on clear bottom, white-walled plates (Greiner Bio-One CELLSTAR 96-well, White, Cell Culture- Treated, Flat-Bottom Microplate, 655098) at 40, 000 cells/well in Leibovitz’s L-15 Medium no phenol red (ThermoFisher, 21083-027), which supports cell growth in environments without CO2 equilibration, supplemented with 10% FBS and substrate Nano-Glo Endurazine Live Cell Substrate (Promega, N2570). ..



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